| 1883 | Bishop Thomas Jagger, first chairman of the Board of Trustees; Mrs. Alexander McGuffey, first president, of the Board of Lady Managers |
| October 1, 1883 | The Home for Sick Children opens in Cincinnati, the city's first pediatric hospital. The Home remained open until 1887 |
| November 10, 1883 | Articles of Incorporation signed for the creation of the Protestant Episcopal Hospital |
| 1884 | First physicians at Children's Hospital: William Carson, N.P. Dandridge, D.S. Connor, W.A. Dunn |
| January 1, 1884 | First Donation Day, raising $2,945 |
| January 16, 1884 | Co-Operative Society holds its first meeting |
| March 1884 | The Hospital of the Protestant Episcopal Church officially opens |
| 1886 | First outpatient clinic at Children's Hospital |
| 1887-1905 | Bishop Boyd Vincent, vice president, Board of Trustees |
| A.G. Rose, DDS, added to the medical staff | |
| November 23, 1887 | The second hospital building, located on North Main Street in Mount Auburn, officially opens (gift of J. Josiah and Thomas J. Emery) |
| 1888 | Cincinnati Children's purchases burial lot at Spring Grove Cemetery |
| July 1889 | Mrs. A. Eliza Gordon, first Governess, and teacher |
| 1894 | Mrs. Henry Probasco, second president, Board of Lady Managers |
| September 1, 1894 | Eliza Dickey, first Superintendent |